Monkey Electrophysiological and Human Psychophysical Responses to Mutants of the Sweet Protein Brazzein: Delineating Brazzein Sweetness
نویسندگان
چکیده
منابع مشابه
Monkey electrophysiological and human psychophysical responses to mutants of the sweet protein brazzein: delineating brazzein sweetness.
Responses to brazzein, 25 brazzein mutants and two forms of monellin were studied in two types of experiments: electrophysiological recordings from chorda tympani S fibers of the rhesus monkey, Macaca mulatta, and psychophysical experiments. We found that different mutations at position 29 (changing Asp29 to Ala, Lys or Asn) made the molecule significantly sweeter than brazzein, while mutations...
متن کاملBrazzein a small, sweet protein: discovery and physiological overview.
For many years, only small molecules were considered capable of inducing a sweet taste (cf. Inglett, 1974). This idea was shattered when miraculin from the miracle fruit of Synsepalum dulcificum was shown to have (Brouwer et al., 1968; Kurihara and Beidler, 1968) a mol. wt of 24 600. The search for sweeteners was no longer limited to small molecules and resulted within a few years in the discov...
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The demand for non-calorigenic protein-based sweeteners with favorable taste properties is high. The optimal design of such sweeteners requires knowledge about structure–function relationships and the identification of chemical entities that trigger the sweetness response. Among the known, naturally occurring, sweet-tasting proteins, brazzein has properties that make it particularly attractive ...
متن کاملInsights into Carbon Content in Examining the Effect of Mutation in Brazzein, a Sweet Tasting Protein
Brazzein is a small sweet testing protein isolated from the fruit of the African plant, Pentadiplandra brazzeana Baillon with potential of replacement of carbohydrate sweeteners. Carbon content analysis was used to examine the effect of mutation brazzein’s two regions at residues 29–33 and 39–43 with residue 36 reported to be important in sweet tasting of the protein. Analysis for local carbon ...
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Sugar, the most popular sweetener, is essential in daily food. However, excessive sugar intake has been associated with several lifestyle-related diseases. Finding healthier and more economical alternatives to sugars and artificial sweeteners has received increasing attention to fulfill the growing demand. Brazzein, which comes from the pulp of the edible fruit of the African plant Pentadipland...
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ژورنال
عنوان ژورنال: Chemical Senses
سال: 2003
ISSN: 1464-3553
DOI: 10.1093/chemse/28.6.491